Coding

Part:BBa_I714002

Designed by: Yang Yifan   Group: iGEM07_Peking   (2007-05-30)


traI of F plasmid

Relaxase/helicase of the F plasmid. Bind to single strand DNA at the oriT region of the F plamid to nick and DNA open.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 127
    Illegal PstI site found at 2203
    Illegal PstI site found at 2684
    Illegal PstI site found at 3154
    Illegal PstI site found at 3307
    Illegal PstI site found at 3925
    Illegal PstI site found at 4720
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 127
    Illegal PstI site found at 2203
    Illegal PstI site found at 2684
    Illegal PstI site found at 3154
    Illegal PstI site found at 3307
    Illegal PstI site found at 3925
    Illegal PstI site found at 4720
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 127
    Illegal PstI site found at 2203
    Illegal PstI site found at 2684
    Illegal PstI site found at 3154
    Illegal PstI site found at 3307
    Illegal PstI site found at 3925
    Illegal PstI site found at 4720
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 127
    Illegal PstI site found at 2203
    Illegal PstI site found at 2684
    Illegal PstI site found at 3154
    Illegal PstI site found at 3307
    Illegal PstI site found at 3925
    Illegal PstI site found at 4720
    Illegal NgoMIV site found at 100
    Illegal NgoMIV site found at 2586
    Illegal NgoMIV site found at 3106
    Illegal NgoMIV site found at 4279
    Illegal NgoMIV site found at 4587
    Illegal AgeI site found at 406
    Illegal AgeI site found at 687
    Illegal AgeI site found at 1621
    Illegal AgeI site found at 1905
    Illegal AgeI site found at 2695
    Illegal AgeI site found at 4021
    Illegal AgeI site found at 4858
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None